Workshop II: Augustenberg
ISTA Workshop on Viability and Germination Testing
- Location: Landwirtschaftliches Technologie Zentrum (Agricultural Technology Park) Augustenberg, Germany,
- Date: 10 to 13 June 2010,
- Fee: 250 Euro for ISTA-Members and 375 Euro for Non-Members,
- Please notice: the workshop is booked up.
Organiser:
- Dr. Andrea Jonitz, Head of Seed Testing Station Augustenberg,
- Dr. Günter Müller, Chair of ISTA Proficiency Test Committee.
Further information and a preliminary programme will follow soon.
Timetable
Thursday 10 June 2010
- 8:30 - 9:00 Registration of participants, technical and organizational matters,
- 9:00 - 9:30 Lecture: Germination of Monocots,
Ronnie Don, - 9:30 - 10:00 Exercise: PHordeum vulgare, germination part I,
Günter Müller, - 10:00 - 10:30 Coffee break,
- 10:30 - 10:45 Lectures: Tetrazolium of Monocots, practical instructions,
Steffi Krämer, - 10:45 - 11:30 Exercise: Tetrazolium of Monocots, preparation of Hordeum and Oryza,
Steffi Krämer, - 11:30 - 12:00 Lectures: Physiological aspects of Dormancy in seed testing,
Norbert Leist, - 12:00 - 13:00 Lunch,
- 13:00 - 13:45 Lectures: Determination of Viability by the Topographical Tetrazolium Test,
Norbert Leist, - 13:45 - 14:30 Exercise: Hordeum vulgare, Tetrazolium evaluation,
Steffi Krämer - 14:30 - 14:45 Excercise: Hordeum vulgare, germination part II evaluation of fresh seeds,
Günter Müller - 14:45 - 15:15 Coffee break
- 15:15 - 16:00 Exercise: Oryza sativa, germination,
Günter Müller - 16:00 - 16:45 Exercise: Oryza sativa, Tetrazolium evaluation,
Steffi Krämer.
Friday 11 June 2010
- 8:30 - 9:15 Lecture: Chemistry of Tetrazolium salts,
Norbert Leist, - 9:15 - 9:45 Exercise: Lolium multiflorum, Tetrazolium preparation,
Steffi Krämer, - 9:45 - 10:00 Discussion: Items brought up by the participants
- 10:00 - 10:30 Coffee break
- 10:30 - 11:15 Exercise: Panicum maximum, Tetrazolium preparation,
Steffi Krämer, - 11:15 - 12:00 Lectures: Physiology and Evaluation of Germination tests,
Ronnie Don, - 12:00 - 13:00 Lunch
- 13:00 - 14:00 Exercise: Lolium multiflorum, Germination evaluation,
Günter Müller, - 14:00 - 14:30 Lecture: Vigour testing by the topographical Tetrazolium test,
Norbert Leist, - 14:30 - 15:00 Coffee break,
- 15:00 - 15:30 Exercise: Medicago, Selection and Tetrazolium preparation,
Steffi Krämer, - 15:30 - 16:30 Exercise: Lolium multiflorum, TEZ evaluation,
Steffi Krämer, - 16:30 - 17:00 Lecture: Statistical aspects of Germination and Tetrazolium testing,
Ronnie Don.
Saturday 12 June 2010
- 8:30 - 9:15 Exercise: Brachiaria brizantha, Tetrazolium preparation,
Steffi Krämer, - 09:15 - 10:00 Exercise: Brachiaria brizantha, Germination evaluation,
Günter Müller, - 10:00 - 10:30 Coffee break,
- 10:45 - 11:30 Lecture: Comparative Evaluation of Germination and Viability in Monocots,
Norbert Leist, Ronnie Don - 11:30 - 12:15 Exercise: Germination Panicum maximum???,
Günter Müller, - 12:15 - 13:15 Lunch,
- 13:15 - 13:45 Lecture: German Occupation by the Allied Forces in 1945 - the Outset of Worldwide Dissemination of Tetrazolium Testing,
Adolf M. Steiner - 13:45 - 14:15 Exercise: Brachiaria brizantha, Tetrazolium evaluation,
Steffi Krämer - 14:15 - 14:45 Lecture: Germination of Dicots,
Ronnie Don, - 14:45 - 15:15 Coffee break,
- 15:15 - 16:00 Exercise: Medicago Germination evaluation, Trifolium germination demonstration,
Günter Müller, - 16:00 - 16:30 Exercise: Medicago, Tetrazolium evaluation;
Steffi Krämer - 16:30 - 17:00 Lecture: Quality assurance in germination and viability testing,
Ronnie Don
Sunday 13 June 2010
- 8:30 - 9:00 Lecture: Presentation of the ISTA,
Norbert Leist, - 9:00 - 9:30 Exercise: Pisum TEZ evaluation and Vicia faba demonstration,
Steffi Krämer, - 9:30 - 10:00 Discussion: Items brought up by the participants
- 10:00 - 10:30 Coffee break,
- 10:30 - 11:15 Exercise: Vicia faba, Germination evaluation,
Günter Müller, - 11:15 - 11:45 Lecture: Work of ISTA Germination Committee and Handbook,
Ronnie Don, - 11:45 - 12:30 Exercise: Portulacca grandiflora, Germination evaluation,
Günter Müller, - 12:30 - 13:30 Lunch,
- 13:45 - 14:30 Lecture: Germination procedure and visit of the seed testing lab Augustenberg,
Andrea Jonitz, - 14:30 - 15:15 Lecture: Comparative Evaluation of Germination and Viability in Dicots,
Norbert Leist / Ronnie Don, - 15:15 - 16:00 Lecture: Final discussion,
- 16:00 - 16:30 Lecture: Closing Ceremony.




