Workshop I: Hanover
ISTA Workshop on Species and Variety Testing / Proteinelectrophoresis
Federal Plant Variety Office in Hanover
- Location: Bundessortenamt (Federal Plant Variety Office), Hanover, Germany
- Date: 11 to 13 June 2010,
- Fee: 250 Euro for ISTA-Members and 375 Euro for Non-Members.
Organiser
- Gabriele Kerschbaumer, Provisional Head of the Laboratory Section
- Cornelia Tepper, Senior Analyst
- Nora-Sophie Schmidt, Federal Plant Variety Office
- Dr. Berta Killermann, Chair of the ISTA Variety Committee
Timetable
Friday, 11 June 2010
PAGE- Methods for Triticum, Hordeum, Avena, Pisum, Lolium and others (xTriticosecale, Poa, ...)
A(cid)-PAGE, SDS-PAGE, different Methods of Native PAGE
- 08:30: Registration of participants
- 09:00: Opening of the Workshop
- Addresses of welcome
- Presentation of the Federal Plant Variety Office
- Introduction of participants and staff members
- 10:15: Coffee break
- 10:45: Theory of PAGE A(cid)-PAGE, SDS-PAGE, Native PAGE (pH 7,9, pH 8,9)
- 11:45: Practice:
- Casting of Polyacrylamide gels
- Sample preparation: Triticum, Hordeum, Triticosecale, Avena
- Demonstration of Sample preparation: Pisum, Zea mays
- 12:45: Lunch
- 14:00: Practice:
- Sample preparation: Lolium, Poa
- Casting of Polyacrylamide gels
- A-PAGE E-run and staining
- SDS-PAGE E-run and staining
- 16:00: Coffee break
- 16:30: Practice /Demonstration:
- A-PAGE E-run and staining
- SDS-PAGE E-run and staining
- Native PAGE (pH 7,9 and pH 8,9)
- 18:00 to 18:30: Questions and Discussion
Saturday, 12 June 2010
Isoelectric Focussing (IEF) for Zea mays, Helianthus annuus
Preliminaries for Starch Gel Electrophoresis (SGE)
- 08:30: Theory of IEF
- 09:00: Practice: IEF (Start of E-run)
- 09:15: Demonstration:
- Casting of IEF gels
- Casting of Starch gels
- 10::15: Practice: IEF (Stop of E-run)
- 10.45: Coffee break
- 11::15: Practice: Destaining and Fixation of PAGE Gels
- 11.45: Questions and discussion
- 12:30: Lunch
- 13:30: Excursion
- 19:00: Official Dinner
Sunday, 13 June 2010
SGE for Zea mays, Brassica, Beta, Helianthus annuus
- 09:00: Theory of Starch gel electrophoresis (SGE)
- 09:30: Practice: SGE - Start of E- run
- 10:00: Practice: SGE – preparation of staining solutions
- 10:30: Coffee break
- 11:00: Time for questions, discussion or deepening of special issues (individual demands can be taken into account)
- 12:00: Lunch
- 13:30: Practice: SGE - End of E-run, slicing, staining the slices, Fixation of gels
- 14:30: Coffee break
- 15:00: Presentation of ISTA and the Variety Committee (activities, tasks and goals, search for co-workers, what should be done in the future? where should be the main focus? which crops should be analysed?)
- 16:30: Final Discussion on several aspects on Species and Variety Testing.
Conclusion
If there is enough time in between we can give further theoretical lectures about Species and Variety Testing (i.e. Objectives of Species and Variety Testing, Overview about conventional methods for Species and Variety Testing, Comparison of UPOV and ISTA).
