Workshop III: Oberschleißheim
Bavarian State Office for Health and Food Safety
ISTA Workshop on GMO Testing
- Location: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (Bavarian State Office for Health and Food Safety) Oberschleißheim, Germany,
- Date: 8 to 12 June 2010,
- Fee: 450 Euro for ISTA Members and 675 Euro for Non-Members,
- Deadline for registrations is 15 May 2010, register here.
Organiser
- Dr. Ulrich Busch, Head of the biomolecular unit
- Benjamin Kaufman, Member of the ISTA GMO Task Forc
Accomodation
"Blauer Karpfen" in Oberschleißheim, www.hotel-blauer-karpfen.de. The rooms are pre-reserved for the following period: 7 to 12 June 2010.
Prices for single room 75 Euro, price for double room 95 Euro.
Tentative Work-Programme
Tuesday, 8th June, 2010
08:00 - 08:15 Opening and introductions
08:15 - 08:45 Introduction to GMO testing & workshop overview (Beni Kaufman)
Testing Plans
(Jean-Louis Laffont)
- 08:45 Theory 1: Theory and basic concepts
- 09:30 Coffee break
- 10:00 Qualitative testing plans – Introduction to Seedcalc
- 12:30 Lunch
- 13:30 Theory 2: Testing plans 2 - Quantitative testing plans
- 14:30 Computer exercises
- 16:00 End of the day one
Wednesday, 9th June, 2010
From Seed To DNA
(Beni Kaufman)
- 08:00 Theory: Sample Preparation: 1. DNA Extractions
- 09:00 Theory: Sample Preparation: 2. DNA quantification, normalization, and sample tracking
- 10:30 Experimental: DNA extraction using NucleoSpin method
- 12:30 Lunch
- 13:30 Experimental: DNA visualization, quantification and normalization
- 16:00 End of day 2 (lab work here may require for additional time)
Thursday, 10th June, 2010
The Poylmerase Chain Reaction
(Cheryl Dollard, Bruno Zaccomer, Jean-Louis Laffont)
- 08:00 Theory: Introduction to PCR (Cheryl Dollard)
- 09:00 Theory: PCR for GMO testing: Definitions and practices (Bruno Zaccomer)
- 10:00 Coffee break
- 10:30 Experimental: Qualitative PCR set -up
- 12:30 Lunch
- 13:30 Outing & Official Dinner
Friday, 11th June, 2010
Real-Time PCR
(Bruno Zaccomer, Beni Kaufman, Cheryl Dollard, Jean-Louis Laffont)
- 08:00 Theory: Real-time PCR for GMO quantification (Bruno Zaccomer)
- 09:00 Experimental: Real-time PCR set –up
- 10:00 Coffee break
- 10:30 Experimental: PCR result visualization & and documentation
- 11:30 Theory: ISTA rules for GMO detection (Cheryl Dollard)
- 12:30 Lunch
- 13:30 Experimental/Theory: Real-Time PCR, data and results analysis and interpretation (Jean-Louis Laffont)
- 15:00 Theory: Protein based methods (Cheryl Dollard)
- 16:00 End of day 4
Saturday, 12th June, 2010
Laboratory Best Practices
(Beni Kaufman, Bruno Zaccomer, Cheryl Dollard)
- 09:00 Theory: Assay and process validation (Beni Kaufman)
- 10:00 Coffee break
- 11:00 Theory & discussion: Management and practices unique to the GMO testing lab (Beni Kaufman, Bruno Zaccomer, Cheryl Dollard)
- 12:00 Conclusion
