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Official Adress by Robert Kloos, State Secretary of the Federal Ministry of Food, Agriculture and Consumer Protection

Welcom adress by Eckhard Uhlenberg, Minister for the Environment and Conservation, Agriculture and Consumer Protection of the State of North Rhine-Westphalia

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Workshop III: Oberschleißheim

Sculpture in front of a building
Bavarian State Office for Health and Food Safety

 

ISTA Workshop on GMO Testing

  • Location: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (Bavarian State Office for Health and Food Safety) Oberschleißheim, Germany,
  • Date: 8 to 12 June 2010,
  • Fee: 450 Euro for ISTA Members and 675 Euro for Non-Members,
  • Deadline for registrations is 15 May 2010, register here.

Organiser

  • Dr. Ulrich Busch, Head of the biomolecular unit
  • Benjamin Kaufman, Member of the ISTA GMO Task Forc

Accomodation

"Blauer Karpfen" in Oberschleißheim, www.hotel-blauer-karpfen.de. The rooms are pre-reserved for the following period: 7 to 12 June 2010.

Prices for single room 75 Euro, price for double room 95 Euro.


Tentative Work-Programme

Tuesday, 8th June, 2010

08:00 - 08:15 Opening and introductions

08:15 - 08:45 Introduction to GMO testing & workshop overview (Beni Kaufman)

Testing Plans

(Jean-Louis Laffont)

  • 08:45 Theory 1: Theory and basic concepts
  • 09:30 Coffee break
  • 10:00 Qualitative testing plans – Introduction to Seedcalc
  • 12:30 Lunch
  • 13:30 Theory 2: Testing plans 2 - Quantitative testing plans
  • 14:30 Computer exercises
  • 16:00 End of the day one

Wednesday, 9th June, 2010

From Seed To DNA

(Beni Kaufman)

  • 08:00 Theory: Sample Preparation: 1. DNA Extractions
  • 09:00 Theory: Sample Preparation: 2. DNA quantification, normalization, and sample tracking
  • 10:30 Experimental: DNA extraction using NucleoSpin method
  • 12:30 Lunch
  • 13:30 Experimental: DNA visualization, quantification and normalization
  • 16:00 End of day 2 (lab work here may require for additional time)

 

Thursday, 10th June, 2010

The Poylmerase Chain Reaction

(Cheryl Dollard, Bruno Zaccomer, Jean-Louis Laffont)

  • 08:00 Theory: Introduction to PCR (Cheryl Dollard)
  • 09:00 Theory: PCR for GMO testing: Definitions and practices (Bruno Zaccomer)
  • 10:00 Coffee break
  • 10:30 Experimental: Qualitative PCR set -up
  • 12:30 Lunch
  • 13:30 Outing & Official Dinner

Friday, 11th June, 2010

Real-Time PCR

(Bruno Zaccomer, Beni Kaufman, Cheryl Dollard, Jean-Louis Laffont)

  • 08:00 Theory: Real-time PCR for GMO quantification (Bruno Zaccomer)
  • 09:00 Experimental: Real-time PCR set –up
  • 10:00 Coffee break
  • 10:30 Experimental: PCR result visualization & and documentation
  • 11:30 Theory: ISTA rules for GMO detection (Cheryl Dollard)
  • 12:30 Lunch
  • 13:30 Experimental/Theory: Real-Time PCR, data and results analysis and interpretation (Jean-Louis Laffont)
  • 15:00 Theory: Protein based methods (Cheryl Dollard)
  • 16:00 End of day 4

Saturday, 12th June, 2010

Laboratory Best Practices

(Beni Kaufman, Bruno Zaccomer, Cheryl Dollard)

  • 09:00 Theory: Assay and process validation (Beni Kaufman)
  • 10:00  Coffee break
  • 11:00 Theory & discussion: Management and practices unique to the GMO testing lab (Beni Kaufman, Bruno Zaccomer, Cheryl Dollard)
  • 12:00 Conclusion